Emva kokuqalisa, izinto eziphathekayo zifakwe kwitanki yezinto zokukhenkceza. Inkqubo yokukhenkceza ibandakanya iinkalo ezimbini: okokuqala, inkqubo yokufunxa isusa ukufuma okuthile ngokutsala ivacuum; okwesibini, njengoko izinto ziqhwala, ezinye iimolekyuli zamanzi zinyanzeliswa kumphezulu kwaye ziqhwale. Nje ukuba imfuneko yokukhenkcisa ifezekiswe, inkqubo yokufudumeza iyatshisa kwaye yomise izinto, itsala ivacuum ukuze isuse nakuphi na ukufuma okuseleyo, okuthi emva koko kuthwalwe kumgibe womkhenkce ukuze kube ngumkhenkce, ngaloo ndlela kufezekiswa imfuneko yomkhenkce{2}}.
Umkhenkce-ukomisa kubhekisa kwinkqubo yokususa amanzi okanye ezinye izinyibilikisi kwiimveliso zebhayoloji ezinomkhenkce ngokusebenzisa i-sublimation. I-sublimation ibhekisa kwinkqubo apho isinyibilikisi, esifana namanzi, njengomkhenkce owomileyo, sitshintsha ngokuthe ngqo ukusuka kwinto eqinileyo ukuya kwimeko enerhasi ngaphandle kokudlula kwimeko engamanzi. Imveliso efunyenwe ngomkhenkce-ukomisa ibizwa ngokuba yi lyophilizer, kwaye inkqubo ibizwa ngokuba ngumkhenkce{4}}ukomisa. Ukomisa ngokwemveli kubangela ukuncipha kwezinto kwaye konakalise iiseli. Ngexesha lomkhenkce{7}}lokoma, ulwakhiwo lwesampulu alutshatyalaliswa ngenxa yokuba amalungu aqinileyo axhaswa ngumkhenkce endaweni yawo. Njengoko umkhenkce uthoba, ushiya imingxuma kwintsalela eyomileyo. Oku kugcina intembeko yebhayoloji kunye nesakhiwo sekhemikhali yemveliso kunye nomsebenzi wayo. Kwilabhoratri, umkhenkce{11}}ukomisa kunemisebenzi emininzi eyahlukeneyo kwaye iyimfuneko kwizicelo ezininzi zemichiza ephilayo nakwizamayeza. Isetyenziselwa ukufumana imathiriyeli yebhayoloji enokugcinwa ixesha elide, elifana neenkcubeko zebhayoloji, iienzymes, igazi, kunye namayeza, igcina hayi kuphela uzinzo{13} lwexesha elide kodwa kunye nomsebenzi wazo wendalo kunye nokwakheka. Ngenxa yesi sizathu, i-lyophilization isetyenziselwa ukulungiselela iisampulu zethishu kwizifundo zesakhiwo (njenge-electron microscopy). I-Lyophilization iphinda isetyenziswe kuhlalutyo lweekhemikhali, ukubonelela ngeesampuli ezomileyo okanye iisampulu ezigxininisiweyo zokwandisa uvakalelo lokuhlalutya. I-Lyophilization izinzisa iisampulu zesampulu ngaphandle kokuguqula ukwakheka kweekhemikhali, iyenza ibe luncedo olufanelekileyo lokuhlalutya. I-Lyophilization ingenzeka ngokwemvelo. Ngaphantsi kweemeko zendalo, le nkqubo icotha kwaye ayiqikeleleki. Ngokuphuhliswa kweenkqubo ze-lyophilization, amanyathelo amaninzi aphuculwe kwaye ahlanjululwa, ukukhawuleza le nkqubo.
